Coding
Part:BBa_K1319004:Design
Designed by: Florian Gohr, Michael Osthege Group: iGEM14_Aachen (2014-09-25)
TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part does not contain start or stop codons, because it was designed for RFC25 compability
Source
The part was codon optimized using the genomic amino acid sequence, designed for RFC25 and mutated S219V for anti-self cleavage properties.